[1]邬波,柳椰,马旭,等.3D打印胶原/羟基磷灰石支架对骨髓间充质干细胞成骨分化的作用研究[J].中华老年骨科与康复电子杂志,2020,(03):123.[doi:10.3877/cma.j.issn.2096-0263.2020.03.001]
 Wu Bo,Liu Ye,et al.Effect of 3D printed collagen / hydroxyapatite scaffold on osteogenic differentiation of bone marrow mesenchymal stem cells[J].Chin J Geriatr Orthop Rehabil(Electronic Edition),2020,(03):123.[doi:10.3877/cma.j.issn.2096-0263.2020.03.001]
点击复制

3D打印胶原/羟基磷灰石支架对骨髓间充质干细胞成骨分化的作用研究()
分享到:

中华老年骨科与康复电子杂志[ISSN:1674-3911/CN:11-9292/R]

卷:
期数:
2020年03期
页码:
123
栏目:
基础研究
出版日期:
2020-06-05

文章信息/Info

Title:
Effect of 3D printed collagen / hydroxyapatite scaffold on osteogenic differentiation of bone marrow mesenchymal stem cells
作者:
邬波12柳椰1马旭12智春升3杜明昌1翟良全1杨政博1王佳媛2王译晗2
110044 沈阳市骨科医院关节外科1;110044 辽宁省骨关节病重点实验室2;110044 沈阳市骨科医院骨病肿瘤科3
Author(s):
Wu Bo1 2 Liu Ye1 Ma Xu1 2 Zhi Chunsheng3 Du Mingchang1 Zhai Liangquan1 Yang Zhengbo1 Wang Jiayuan 2 Wang Yihan 2
1Department of Joint Surgery, Shenyang Orthopaedic Hospital, Shenyang 110044, China; 2Key Laboratory of Osteoarthropathy of Liaoning Province, Shenyang 110044, China; 3Department of Orthopaedic and Oncology, Shenyang Orthopaedic Hospital, Shenyang 110044, China
关键词:
胶原 羟基磷灰石 间充质干细胞 成骨分化
Keywords:
Collagen Hydroxyapatite Mesenchymal Stem Cells Osteogenic differentiation
DOI:
10.3877/cma.j.issn.2096-0263.2020.03.001
文献标志码:
A
摘要:
目的 探讨3D打印胶原/羟基磷灰石支架对骨髓间充质干细胞成骨分化的作用。方法 分离SPF级雄性SD大鼠骨髓间充质干细胞,实验分为:对照组、浸提组、诱导组及浸提诱导组,MTT法检测对照组,浸提组的细胞增殖情况,对比分析各组细胞的碱性磷酸酶(ALP)活性,对各组细胞诱导培养17天后进行茜素红染色,观察钙结节染色情况。结果 MTT结果显示,浸提组与对照组在不同时间点的OD值比较,差异无统计学意义(P>0.05),ALP 活性结果显示,不同时间点各组与对照组相比,差异均有统计学意义(P<0.05);不同时间点浸提诱导组与浸提组相比,差异均有统计学意义(P<0.05);诱导组在48 h及72 h与浸提组相比,差异均有统计学意义(P<0.05)。茜素红染色结果显示,对照组细胞无钙结节点,浸提组及诱导组镜下肉眼可明显观察到红色区域染色,镜下观察可见钙结节点,浸提诱导组所产生的钙结节点的数量、大小以及染色的颜色深度均明显优于其他各组。结论 3D打印胶原/羟基磷灰石支架具有生物相容性好,可促进BMSCs向成骨分化,对细胞毒性低等特点,适宜用作骨缺损的治疗。
Abstract:
Objective To investigate the effect of 3D printed collagen / hydroxyapatite scaffold on osteogenic differentiation of bone marrow mesenchymal stem cells. Methods Rat BMSCs were isolated from rat bone marrow. Cell proliferation and differentiation were detected by MTT and ALP activity. After inducing cell culture for 17 days, cells in each group were stained with alizarin red to observe the staining of calcium nodules. Results The MTT results showed that there was no significant difference in OD between the extraction group and the control group at different time point (P>0.05). ALP activity results showed that the extraction group had significant differences compared with the control group at different time point (P<0.05); the extraction induction group had significant differences at different time point compared with the extraction group (P<0.05); At 48 h and 72 h, the group had significant differences compared with the extraction group (P<0.05). Alizarin red staining showed that the cells in the control group had no calcium knot nodes. The red area staining was clearly observed under the microscope in the extraction group and the induction group, and the calcium knot nodes were observed under the microscope. The number, size and color depth of the knot nodes were significantly better than those of the other groups. Conclusion The scaffold has good biocompatibility, can promote the differentiation of BMSCs to osteogenesis, and has low cytotoxicity. It is suitable for the treatment of bone defect.

参考文献/References:

1 闫昭, 曹晓瑞, 孙孟帅, 等. 全膝关节翻修术中骨缺损的处理研究进展 [J]. 中华关节外科杂志: 电子版, 2018, 12(3): 84-89.2 危小东, 陈永岗, 刘佳, 等. 全膝关节置换术中胫骨平台骨缺损的最新处理进展 [J]. 实用骨科杂志, 2016, 22(12): 1100-1103.3 Sugita T, Aizawa T, Miyatake N, et al. Preliminary results of managing large medial tibial defects in primary total knee arthroplasty:autogenous morcellised bone graft [J]. Int Orthop, 2017, 41(5): 931-937.4 Vasso M, Beaufils P, Cerciello S, et al. Bone loss following knee arthroplasty:potential treatment options [J]. Arch Orthop Trauma Surg, 2014, 134(4): 543-553.5 叶一林, 朱天岳, 柴卫兵, 等. 混合植骨技术结合髋臼加强杯或钛网杯重建髋臼严重骨缺损 [J]. 中华骨科杂志, 2012, 32(9): 830-836.6 Venkatesan J, im S-K. Nano-Hydroxyapatite composite bioma- terials for bone tissue engineering-A review [J]. J Biomed Nano-technol, 2014, 10: 3124-3140.7 Tsukada S, Wakui M, Matsueda M. Metal block augmentation for bone defects of the medial tibia during primary total knee arthroplasty [J]. J Orthop Surg Res, 2013: 36.8 Chen Y, Zheng Z, Zhou R, et al. Developing a strontium-releasing graphene oxide/collagen-based organic-inorganic nanobiocomposite for large bone defect regeneration via MAPK signaling pathway [J]. ACS Applied Materials & Interfaces, 2019.9 Chekmazov IA, Riabov AL, Skalozub OI, et al. Biocomposite nanostructured materials for the bone defects filling by osteomyelitis [J]. Khirurgiia (Mosk), 2013 (8): 56.10 赵畅, 曾参军, 蔡道章. 髋膝关节翻修术中的关键问题及3D打印应对之策 [J]. 中华关节外科杂志:电子版, 2017, 11(3): 284-288.11 王荣诗, 谭伦, 周欣, 等. 3D打印技术在伴骨缺损髋关节置换(翻修)术中的应用 [J]. 临床骨科杂志, 2019, 22(2): 169-171.12 Prashad R, Yasar O. Three-Dimensional scaffold fabrication with inverse photolithography [J]. MRS Advances, 2016, 2(19/20): 1071-1075.13 Blakeney BA, Tambralli A, Anderson JM, et al. Cell infiltration and growth in a low density,uncompressed three-dimensional electrospun nanofibrous scaffold [J]. Biomaterials, 2011, 32(6): 1583-1590.14 Adnan H , Sukyoung K , Man-Woo H , et al. BMP-2 Grafted nHA/PLGA Hybrid Nanofiber Scaffold Stimulates Osteoblastic Cells Growth [J]. Biomed Research International, 2015, 2015: 1-12.15 Bianco JER , Rosa RG , Castillo AC , et al. Characterization of a novel decellularized bone marrow scaffold as inductive environment for hematopoietic stem cells [J]. Biomaterials Science, 2019, 7(1).16 Liu X, Zhu C, Li Y, et al. The preparation and in vitro evaluations of a nanoscaled injectable bone repair material [J]. J Nanomater, 2015 (4): 1-8.17 Mandrycky C, Wang Z, Kim K, et al. 3D bioprinting for engineering complex tissues [J]. Biotechnol Adv, 2016, 34(4): 422-434.18 El-Ghannam E, Ahmed. Bone Reconstruction:from bioceramics to tissue engineering [J]. Expert Rev Med Devices, 2005, 2(1): 87-101.19 W?odarski KH, W?odarski PK, Galus R. Bioactive composites for bone regeneration [J]. Ortop Traumatol Rehabil, 2008, 10(3): 201-210.20 Rahman MS, Rana MM, Lucas-Sebastian S, et al. Fabrication of biocompatible porous scaffolds based on hydroxyapatite/collagen/chitosan composite for restoration of defected maxillofacial mandible bone [J]. Progress in Biomaterials, 2019, 8(3): 137-154.21 邬波, 柳椰, 马旭, 等. 3D打印个性化导航模板在全膝关节置换术中的应用 [J]. 中国骨与关节损伤杂志, 2017, 32(2): 148-151.22 Qiu YY, Yan CH, Chiu KY, et al. Review article:bone defect classifications in revision total knee arthroplasty [J]. J Orthop Surg (Hong Kong), 2011, 19(2): 238-243.23 Luo W, Huang L, Liu H, et al. Customized knee prosthesis in treatment of giant cell tumors of the proximal tibia: application of 3-dimensional printing technology in surgical design [J]. Med Sci Monit, 2017, 23: 1691-1700. 24 Wang Xing, Xing Helin, Zhang Guilan, et al. Restoration of a critical mandibular bone defect using human alveolar bone-derived stem cells and porous nano-HA/Collagen/PLA scaffold [J]. Stem Cells International, 2016: 1-13.25 Dewey MJ, Johnson EM, Weisgerber DW, et al. Shape-fitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells [J]. J Mech Behav Biomed Mater, 2019, 95: 21-33.

备注/Memo

备注/Memo:
辽宁省自然科学基金指导计划(20180550795)
更新日期/Last Update: 2020-07-07