[1]贺方正,吴涛,廖长胜,等.创伤后骨关节炎模型大鼠血浆中microRNA特征组学研究[J].中华老年骨科与康复电子杂志,2025,(05):257-270.[doi:10.3877/cma.j.issn.2096-0263.2025.05.001]
　He Fangzheng,Wu Tao,Liao Changsheng,et al.MicroRNA signature omics study in the blood of rats with post-traumatic osteoarthritis model[J].Chin J Geriatr Orthop Rehabil(Electronic Edition),2025,(05):257-270.[doi:10.3877/cma.j.issn.2096-0263.2025.05.001]
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创伤后骨关节炎模型大鼠血浆中microRNA特征组学研究()
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中华老年骨科与康复电子杂志[ISSN:1674-3911/CN:11-9292/R]

卷:
期数:
2025年05
页码:
257-270
栏目:
基础研究
出版日期:
2025-10-05

文章信息/Info

Title:
MicroRNA signature omics study in the blood of rats with post-traumatic osteoarthritis model
作者:
贺方正; 吴涛; 廖长胜; 李锡勇; 牛萌煊; 韩鹏飞
046000 长治医学院附属和平医院骨科
Author(s):
He Fangzheng;  Wu Tao;  Liao Changsheng;  Li Xiyong;  Niu Mengxuan;  Han Pengfei.
Department of Orthopaedics, Heping Hospital Affiliated to Changzhi Medical College, Changzhi 046000, China
关键词:
创伤后骨关节炎;  MicroRNAs;  生物标志物;  组学
Keywords:
Post-traumatic osteoarthritis;  MicroRNAs;  Biomarkers;  Omics
DOI:
10.3877/cma.j.issn.2096-0263.2025.05.001
文献标志码:
A
摘要:
目的 本研究着重研究了创伤后骨关节炎（PTOA）大鼠模型血浆中微小核糖核酸（MicroRNA）的表达谱差异，并通过生物信息学分析试图阐明microRNA在PTOA疾病发生发展过程中的作用。方法 从9只两个月大的雌性Sprague-Dawley大鼠中采集血液样本，并对微小核糖核酸（miRNAs）进行测序。这些大鼠被分为三组：正常对照组（K组：无干预），手术诱导的轻度创伤后骨关节炎组（H组：改良Hulth模型手术后三周），以及手术诱导的重度创伤后骨关节炎组（J组：改良Hulth模型手术后五周）。利用实时荧光定量聚合酶链式反应（PCR）鉴定并验证了差异表达。确定了三组间的miRNA差异表达数据，并通过生物信息学分析进行了靶基因预测和通路分析。通过重组人白细胞介素1β（IL-1β）诱导建立了人膝关节骨关节炎样软骨细胞系。并利用逆转录聚合酶链式反应（RT-PCR）验证了差异表达的miRs是否在人膝关节软骨细胞和人膝关节骨关节炎样软骨细胞中差异表达。结果 在H组和K组之间，miR-6315、miR-143-5p、miR-150-5p和miR-301b-3p的表达存在差异。其中，miR-6315、miR-150-5p和miR-301b-3p的表达上调，而miR-143-5p的表达下调。在J组和K组之间，miR-6315的表达上调且存在差异。然而，在H组和J组之间，miR-511-3p和miR-301a-3p的表达上调且存在差异。此外，在K组和H组（早期阶段）之间，miR-6315、miR-143-5p、miR-150-5p和miR-301b-3p的表达存在差异，这表明它们可以作为大鼠PTOA关节损伤的生物标志物。在J组中，miR-6315的表达也上调，这与上述结果一致。因此，miR-511-3p和miR-301a-3p可作为区分轻度和重度PTOA的生物标志物。其中，miR-143-5p、miR-150-5p和miR-301b-3p在人的膝关节骨关节炎软骨细胞和人的膝关节软骨细胞中也存在差异表达。特别是，miR-150-5p和miR-301b-3p的表达上调且具有统计学显著性。结论 miR-6315、miR-143-5p、miR-150-5p、miR-301b-3p可用作大鼠PTOA关节损伤生物标志物。现在有必要对人体组织中的这些特定分子进一步进行研究，以检查它们作为人类PTOA损伤生物标志物的潜在适用性。
Abstract:
Objective This study focused on analyzing the differential expression profiles of microRNAs in the plasma of a rat model with post-traumatic osteoarthritis (PTOA) and aimed to elucidate the role of microRNAs in the pathogenesis and progression of PTOA through bioinformatics analysis. Methods Blood samples were collected from nine two-month-old female Sprague-Dawley rats, and microRNAs (miRNAs) were sequenced. The rats were divided into three groups: a normal control group (Group K: no intervention), a mild PTOA group induced by surgery (Group H: modified Hulth model at three weeks post-surgery), and a severe PTOA group induced by surgery (Group J: modified Hulth model at five weeks post-surgery). Differential miRNA expression was identified and validated using quantitative real-time polymerase chain reaction (qPCR). Bioinformatics analysis was performed to predict target genes and analyze associated pathways. A human knee osteoarthritis-like chondrocyte model was established by stimulating chondrocytes with recombinant human interleukin-1β (IL-1β). Reverse transcription PCR (RT-PCR) was used to verify whether the differentially expressed miRNAs were also differentially expressed in human knee chondrocytes and human knee osteoarthritis-like chondrocytes. Results Differential expression was observed between Group H and Group K for miR-6315, miR-143-5p, miR-150-5p, and miR-301b-3p. Among these, miR-6315, miR-150-5p, and miR-301b-3p were upregulated, while miR-143-5p was down regulated. Between Group J and Group K, miR-6315 was significantly upregulated. However, between Group H and Group J, miR-511-3p and miR-301a-3p were upregulated and showed differential expression. Furthermore, in the comparison between Group K and Group H (early stage), miR-6315, miR-143-5p, miR-150-5p, and miR-301b-3p exhibited differential expression, suggesting their potential as biomarkers for joint injury in rat PTOA. In Group J, miR-6315 was also upregulated, consistent with the above findings. Thus, miR-511-3p and miR-301a-3p may serve as biomarkers to distinguish between mild and severe PTOA. Notably, miR-143-5p, miR-150-5p, and miR-301b-3p were differentially expressed in human knee osteoarthritis chondrocytes compared to normal human knee chondrocytes, with miR-150-5p and miR-301b-3p showing significant upregulation. Conclusion miR-6315, miR-143-5p, miR-150-5p, and miR-301b-3p may serve as biomarkers for joint injury in rat PTOA. Further investigation in human tissues is warranted to evaluate their potential applicability as biomarkers for PTOA in humans.

备注/Memo

备注/Memo:
基金项目：长治医学院博士科研启动基金（BS202004）
更新日期/Last Update: 2026-05-18