[1]傅志炜 伍锦涛 张术涛 岳冰.术前规律性运动可预防及缓解膝关节假体周围感染[J].中华老年骨科与康复电子杂志,2026,(01):15-24.[doi:10.3877/cma.j.issn.2096-0263.2026.01.003]
　Fu Zhiwei,Wu Jintao,Zhang Shutao,et al.Preoperative regular exercise can prevent and mitigate knee periprosthetic joint infection[J].Chin J Geriatr Orthop Rehabil(Electronic Edition),2026,(01):15-24.[doi:10.3877/cma.j.issn.2096-0263.2026.01.003]
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术前规律性运动可预防及缓解膝关节假体周围感染()
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中华老年骨科与康复电子杂志[ISSN:1674-3911/CN:11-9292/R]

卷:
期数:
2026年01期
页码:
15-24
栏目:
术后感染
出版日期:
2026-02-05

文章信息/Info

Title:
Preoperative regular exercise can prevent and mitigate knee periprosthetic joint infection
作者:
傅志炜 伍锦涛 张术涛 岳冰
200127 上海交通大学医学院附属仁济医院骨关节外科
Author(s):
Fu Zhiwei;  Wu Jintao;  Zhang Shutao;  Yue Bing.
Department of Orthopaedic Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
关键词:
术前规律性运动;  膝关节假体周围感染;  炎症反应;  骨溶解
Keywords:
Preoperative regular exercise;  Periprosthetic joint infection;  Inflammatory response;  Osteolysis
DOI:
10.3877/cma.j.issn.2096-0263.2026.01.003
文献标志码:
A
摘要:
目的 探讨术前规律性运动对小鼠膝关节假体周围感染的预防和缓解作用。方法 建立C57BL/6雄性小鼠膝关节PJI模型，将小鼠分为空白对照组、PJI组和术前规律性运动+PJI组。术前规律性运动+PJI组在PJI模型建立前进行为期6 w的跑台规律性运动干预。术后4 w，通过大体观察、X射线、Micro-CT、Giemsa染色、组织病理学染色（H&E、TRAP、Alcian blue、COL1A1免疫荧光）和qPCR检测，评估PJI的感染负荷、骨溶解、炎症反应及组织修复微环境。结果 与PJI组相比，术前规律性运动+PJI组显著减轻了PJI的感染程度和细菌负荷，缓解了骨溶解（表现为骨膜反应减轻、破骨细胞数量减少、骨微结构参数如BMD、BV/TV等改善），降低了促炎因子（Il1b、Il6、Tnf、Cxcl15、Myd88、Tlr4）的表达水平，同时上调了抗炎因子（Il4、Il10）的表达，并减轻了局部组织炎症浸润和纤维化程度。结论 术前规律性运动可通过减轻感染负荷、缓解骨溶解、控制炎症反应和改善组织修复微环境，有效缓解小鼠PJI的严重程度，为临床预防PJI提供了新的试验依据和潜在策略。
Abstract:
Objective To investigate the preventive and alleviating effects of preoperative regular exercise on periprosthetic knee joint infection in mice. Methods A knee periprosthetic joint infection (PJI) model was established in C57BL/6 male mice. The mice were divided into three groups: a blank control group, a PJI group, and a preoperative regular exercise + PJI group. The preoperative regular exercise + PJI group underwent a 6-week regular treadmill exercise intervention before the PJI model was established. Four weeks postoperatively, the infectious burden of PJI, osteolysis, inflammatory response, and the tissue repair microenvironment were assessed through gross observation, X-ray imaging, Micro-CT analysis, Giemsa staining, histopathological staining (H&E, TRAP, Alcian blue, COL1A1 immunofluorescence), and qPCR detection. Results Compared to the PJI group, preoperative regular exercise significantly reduced the severity of PJI and bacterial load, alleviated osteolysis (as evidenced by reduced periosteal reaction, decreased osteoclast numbers, and improved bone microarchitectural parameters such as BMD and BV/TV), decreased the expression levels of pro-inflammatory factors (Il1b, Il6, Tnf, Cxcl15, Myd88, Tlr4), while concurrently upregulating the expression of anti-inflammatory factors (Il4, Il10), and diminished local tissue inflammatory infiltration and the degree of fibrosis. Conclusions Preoperative regular exercise can effectively alleviate the severity of periprosthetic joint infection (PJI) in mice by reducing infectious burden, mitigating osteolysis, controlling the inflammatory response, and improving the tissue repair microenvironment. This provides new experimental evidence and potential strategies for the clinical prevention of PJI.

备注/Memo

备注/Memo:
基金项目：国家自然科学基金（82172464）
更新日期/Last Update: 2026-05-18