[1]李军 沈姝霖 张荣炜 黄章睿 梁锐明 罗世兴.槲皮素对H2O2诱导骨关节炎软骨细胞氧化损伤的保护作用与机制研究[J].中华老年骨科与康复电子杂志,2026,(02):85-92.[doi:10.3877/cma.j.issn.2096-0263.2026.02.004]
　Li Jun,Shen Shulin,et al.Protective Effect of Quercetin against H2O2-Induced Oxidative Damage in Osteoarthritic Chondrocytes[J].Chin J Geriatr Orthop Rehabil(Electronic Edition),2026,(02):85-92.[doi:10.3877/cma.j.issn.2096-0263.2026.02.004]
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槲皮素对H2O2诱导骨关节炎软骨细胞氧化损伤的保护作用与机制研究()
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中华老年骨科与康复电子杂志[ISSN:1674-3911/CN:11-9292/R]

卷:
期数:
2026年02期
页码:
85-92
栏目:
基础研究
出版日期:
2026-04-05

文章信息/Info

Title:
Protective Effect of Quercetin against H2O2-Induced Oxidative Damage in Osteoarthritic Chondrocytes
作者:
李军1; 2 沈姝霖1; 2 张荣炜1; 2 黄章睿1; 2 梁锐明1; 2; 3 罗世兴1; 4
530021 南宁，广西医科大学再生医学与医用生物资源开发应用省部共建协同创新中心1；530021 南宁，广西组织器官修复医用生物材料工程技术研究中心2；530021 南宁，广西医科大学生命科学研究院3；536000 北海，广西医科大学第九附院医院骨科4
Author(s):
Li Jun1;  2;  Shen Shulin1;  2;  Zhang Rongwei1;  2;  Huang Zhangrui1;  2;  Liang Ruiming1;  2;  3;  Luo Shixing1;  4.
1Collaborative Innovation Centre of Regenerative Medicine and Medical Bio-Resource Development and Application Co-constructed by the Province and Ministry, Guangxi Medical University, Nanning 530021, China; 2Guangxi Engineering Research Centre for Medical Biomaterials of Tissue and Organ Repair, Nanning 530021, China; 3Life Sciences Institute, Guangxi Medical University, Nanning, 530021, China; 4Department of Orthopedics, The Ninth Affiliated Hospital of Guangxi Medical University, Beihai 536000, China
关键词:
槲皮素;  骨关节炎;  氧化应激;  线粒体;  软骨细胞
Keywords:
Quercetin;  Osteoarthritis;  Oxidative Stress;  Mitochondria;  Chondrocytes
DOI:
10.3877/cma.j.issn.2096-0263.2026.02.004
文献标志码:
A
摘要:
目的 研究槲皮素（quercetin, Que）对过氧化氢（H2O2）诱导的骨关节炎（osteoarthritis, OA）软骨细胞氧化损伤的保护作用及相关分子机制。方法 体外分离培养乳鼠关节软骨细胞，随机分为正常对照组、模型组（H2O2诱导）及处理组（Que干预组）。采用 CCK-8法筛选 Que 的安全浓度；利用 Calcein-AM 染色检测细胞活力；以 DCFH-DA、DHE、DAF-FM DA 及 JC-1荧光探针分别检测细胞内ROS、超氧阴离子、NO水平及线粒体膜电位变化；采用免疫荧光和 RT-qPCR 检测炎症因子及基质降解酶的表达。结果 10～40 μg/mL Que 对软骨细胞无明显毒性。与模型组相比，40 μg/mL Que 显著提高了细胞活力（P＜0.05），降低胞内 ROS、超氧阴离子及 NO 水平，并改善 H2O2导致的线粒体膜电位下降（P＜0.05）。同时，Que 可显著下调 MMP-13、IL-6蛋白表达，以及 MMP-3、MMP-13、IL-1β、IL-6 mRNA 的表达（P＜0.05）。结论 槲皮素可通过清除活性氧、抑制 NO 生成、维持线粒体功能，减轻H2O2诱导的软骨细胞氧化应激与炎症反应，对OA软骨细胞具有保护作用，有望成为骨关节炎治疗的天然候选药物。
Abstract:
Objective To investigate the protective effects and related molecular mechanisms of quercetin (Que) against oxidative stress, inflammatory response, and mitochondrial dysfunction induced by hydrogen peroxide H2O2 osteoarthritis (OA) chondrocytes. Methods Articular chondrocytes were isolated from neonatal rats and randomly divided into a normal control group, a model group (H2O2 induced), and a treatment group (Que intervention). The safe concentration of Que was determined by CCK-8 assay. Cell viability was evaluated using Calcein-AM staining. The levels of intracellular ROS, superoxide anion, NO, and mitochondrial membrane potential were detected by DCFH-DA, DHE, DAF-FM DA, and JC-1 fluorescent probes, respectively. The expression of inflammatory factors and matrix-degrading enzymes was measured by immunofluorescence and RT-qPCR. Results Que at 10-40 μg/mL showed no obvious cytotoxicity to chondrocytes. Compared with the model group, 40 μg/mL Que significantly increased cell viability (P<0.05), reduced intracellular levels of ROS, superoxide anion, and NO, and ameliorated the H2O2 induced decrease in mitochondrial membrane potential (P<0.05). Meanwhile, Que significantly downregulated the protein expression of MMP-13 and IL-6 as well as the mRNA expression of MMP-3, MMP-13, IL-1β, and IL-6 (P<0.05). Conclusion Quercetin attenuates H2O2 induced oxidative stress and inflammation in chondrocytes by scavenging reactive oxygen species, inhibiting NO production, and maintaining mitochondrial function. It exerts a protective effect on OA chondrocytes and is expected to be a natural candidate for the treatment of osteoarthritis.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（82360425）；广西科技创新平台计划（桂科 LT2600640002）
更新日期/Last Update: 2026-05-19