[1]张伟丽,罗敏,彭江,等.BMP-2/Smads 信号通路促进骨代谢失衡大鼠骨髓间充质干细胞的成骨分化[J].中华老年骨科与康复电子杂志,2021,(02):65-72.[doi:10.3877/cma.j.issn.2096-0263.2021.02.001]
　Zhang Weili,Luo Min,Peng Jiang,et al.The BMP-2/Smads signaling pathway promote the osteogenic differentiation of mesenchymal stem cells in rat with bone metabolism imbalance[J].Chin J Geriatr Orthop Rehabil(Electronic Edition),2021,(02):65-72.[doi:10.3877/cma.j.issn.2096-0263.2021.02.001]
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BMP-2/Smads 信号通路促进骨代谢失衡大鼠骨髓间充质干细胞的成骨分化()
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中华老年骨科与康复电子杂志[ISSN:1674-3911/CN:11-9292/R]

卷:
期数:
2021年02期
页码:
65-72
栏目:
基础研究
出版日期:
2021-04-05

文章信息/Info

Title:
The BMP-2/Smads signaling pathway promote the osteogenic differentiation of mesenchymal stem cells in rat with bone metabolism imbalance
作者:
张伟丽; 罗敏; 彭江; 赵斌
100039 北京，解放军总医院第三中心住院与病案管理科1，泌尿外科2；100853北京，解放军总医院骨研所3，第三中心骨科4
Author(s):
Zhang Weili1;  Luo Min2;  Peng Jiang3;  Zhao Bin4
1Inpatient and Medical Record Management Division of 3rd Medical Center，Chinese PLA General Hospital, 69 Yong ding Road, Hai dian District, Beijing 100039, China; 2Urology Department of 3rd Medical Center, Chinese PLA General Hospital, 69 Yong ding Road, Hai dian District, Beijing 100039, China; 3Institute of Orthopaedics, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China; 4Orthopaedic Department of 3rd Medical Center, Chinese PLA General Hospital, 69 Yong ding Road, Hai dian District, Beijing 100039, China
关键词:
BMP信号通路;  骨髓间充质干细胞;  骨质疏松;  成骨分化
Keywords:
BMP signaling pathway;  Bone marrow mesenchymal stem cells;  Osteoporosis;  Osteogenic differentiation
DOI:
10.3877/cma.j.issn.2096-0263.2021.02.001
文献标志码:
A
摘要:
目的 本研究旨在探讨BMP-2/Smads信号通路对骨代谢失衡大鼠骨髓间充质干细胞（rBMSCs）成骨分化的影响。方法 切除雌性SD大鼠双侧卵巢，建立大鼠骨代谢失衡模型，即骨质疏松（osteoporosis，OP）模型。通过成骨诱导鉴定rBMSCs的成骨分化。采用脂肪诱导和流式细胞术（FCM）检测脂肪分化和rBMSC表面标志物的表达。将rBMSCs分为空白组、NC组（转染大鼠rBMSCs的阴性对照质粒）、SI-BMP2组（转染大鼠rBMSCs具有低表达BMP-2质粒）和OE-BMP2组（转染大鼠rBMSCs具有高表达BMP-2质粒）。Western blot和定量实时聚合酶链反应（qRT-PCR）检测关键因子和成骨相关因子的表达水平。茜素红染色观察钙化结节的形成。碱性磷酸酶染色测定ALP活性。结果 OP组大鼠体重增加，骨密度下降（均P＜0.01）。OP大鼠rBMSCs具有成骨分化和成脂分化能力，CD44（91.3±2.9%）和CD105（94.8±2.1%）高表达。与空白组相比，OE-BMP2组BMP-2和SMAD1水平升高，钙化结节和ALP阳性染色面积增加（均P＜0.05）。OE-BMP2组RUNX2、OC、OPN表达水平均高于空白组（均P＜0.05）。SI-BMP2组的结果与OE-BMP2组相反。结论 BMP-2激活的BMP信号通路可促进骨代谢失衡大鼠rBMSCs的成骨分化。
Abstract:
Objective The purpose of this study was to investigate the effect of BMP-2/Smads signaling pathway on osteogenic differentiation of bone marrow mesenchymal stem cells (rBMSCs) in rat with bone metabolism imbalance. Methods Ovaries of female SD rats were excised and OP model was established. The osteogenic differentiation of rBMSCs was identified by osteogenic induction. Fat induction and flow cytometry (FCM) were used to detect fat differentiation and the expression of rBMSC surface markers. rBMSCs were divided into blank group, negative control (NC) group (transfected with NC BMP-2 plasmid), SI-BMP2 group (transfected with low expressing BMP-2 plasmid) and OE-BMP2 group (transfected with high expressing BMP-2 plasmid). Western blot and quantitative real-time PCR were used to detect the expression of key factors and osteogenic related factors. The formation of calcified nodules was observed by alizarin red staining. ALP activity was determined by alkaline phosphatase staining. Results In OP group, the weight and bone mineral density of rats increased (P<0.01). rBMSCs of OP rats have the ability of osteogenic differentiation and lipogenic differentiation. CD44 (91.3±2.9%) and CD105 (94.8±2.1%) are highly expressed. Compared with the blank group, the level of BMP-2 and Smad1 in OE-BMP2 group increased, and the area of calcified nodule and ALP positive staining increased (P<0.05). The expression level of Runx2, OC and OPN in OE-BMP2 group was higher than that in blank group (P<0.05). The results of SI-BMP2 group were opposite to those of OE-BMP2 group. Conclusion The BMP signaling pathway activated by BMP-2 can promote the osteogenic differentiation of rBMSCs in OP rats.

备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目（NSFC81472092）
更新日期/Last Update: 2021-05-10